DSG-Ⅰ型生物信息红外肝病治疗仪治疗大鼠非酒精性脂肪肝

DSG-Ⅰ型生物信息红外肝病治疗仪治疗大鼠非酒精性脂肪肝

   世界华人消化杂志 2008年3月28日16(9):935-940 ISSN1009-3079 CN14-1260/R
                                                                          •论著•
窦爱霞，宗蕾，周琨，陈婷，潘浩，张权，李继强，房静远，陆伦根
窦爱霞, 宗蕾，周琨，陈婷，潘浩，张权，李继强，房静远，陆伦根,上海交通大学医学院附属仁济医院消化内科  上海市消化疾病研究所 上海市  200001
窦爱霞，2005级上海交通大学医学院附属仁济医院消化内科硕士，主要从事非酒精性脂肪性肝病发病机制及治疗的研究。
上海市科学技术委员会资助项目，No.054119618
上海市重点学科建设资助项目，No.Y0205
作者贡献分布：陆伦根、窦爱霞、潘浩对此文所作贡献均等；此课题由陆伦根、窦爱霞、张权、李继强及房静远设计；研究过程由窦爱霞、宗蕾、周琨、陈婷及潘浩操作完成；数据分析由宗蕾、潘浩完成；本论文写作由窦爱霞及陆伦根完成
通讯作者：陆伦根,200001,上海交通大学医学院附属仁济医院消化内科，上海市消化疾病研究所.lungenlu1965@yahoo.com
电话：021-53882001           传真：021-63266027
收稿日期：2007-09-16         修回日期：2008-03-08
Experimental study on nonalcoholic fatty liver disease treated by medical instrument on hepatic diseases of type DSG in rats
Ai-Xia Dou, Lei Zong,Kun Zhou,Ting Chen,Hao Pan，Quan Zhang，Ji-Qiang Li， Jing-Yuan Fang，Lun-Gen Lu

Ai-Xia Dou, Lei Zong, Kun Zhou, Ting Chen, Hao Pan，Quan Zhang，Ji-Qiang Li， Jing-Yuan Fang，Lun-Gen Lu. Department of Gastroenterology，Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai JiaoTong University School of Medicine， Shanghai 200001 ,China
Supported by: the science Technology Committee Program of Shanghai, No.054119618, the Shanghai Leading Academic Discipline Project No.Y0205
Correspondence to:Dr. Lun-Gen Lu, Department of Gastroenterology，Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai JiaoTong University School of Medicine，Shanghai 200001 China. lungenlu1965@yahoo.com
Received:2007-09-16   Revised:2008-03-08
Abstract
AIM: To investigate the primary therapeutic efficacy of  the medical instrument on hepatic
diseases of type  I  DSG in treating  nonalcoholic fatty liver disease (NAFLD）of rats．
METHODS: Fifty male SD rats were randomly divided into 5 groups.The control groups (n=20,N1 and N2 group) were fed with normal diet; and the model groups (n=30,M1,M2 and T group) were fed with fat-rich diet consisting of 0.88 standard normal diet+0.10 lard oil +0.02 cholesterol.Therapeutic group (T group) was treated from wk 13 and finished at the wk 20..The  levels of serum alanine aminotransferase (ALT)、aspartate aminotransferase (AST)、gamma-glu-tamyl-transpeptidase (GGT)、alkaline phospha-tase (ALP)、total protein (TP)、triglycerides (TG)、total cholesterol (TC)、low-density lipoprotein (LDL)、and high density lipoprotein (HDL) were measured. The hepatic histologic changes were observed with HE and Masson staining.
RESULTS： At the end of the experiment,compared with those in the normal control group，the liver index (t=8.65,p<0.01)and serum ALT(t=4.65,p<0.01)、AST(t=4.74,p<0.01)、ALP(t=3.56,p<0.01)、TP(t=6.35,p<0.01)、TC(t=8.41,p<0.01)、LDL(t=8.86,p<0.01) levels were increased significantly in the model control group，and the hepatic steatosis (z=-4.135,p<0.01)、inflammation(z=-3.752,p<0.01) and fibrosis (z=-4.156,p<0.01)were more serious. Compared with those in the model control groups，the serum ALT、GGT、ALP、TP、TG、and TC levels were all down-regulated in the therapeutic group without significances, while AST(t=-2.404,p<0.05) level was reduced significantly.The degrees of the hepatic steatosis and inflammation were decreased without statistical significances,but fibrosis degrees were lightened markedly(z=-3.101,p<0.01).
CONCLUSION: The medical instrument on hepatic diseases of type I DSG has some therapeutic effect in treating NAFLD of rats.
Key Words:  Nonalcoholic fatty liver disease; Medical instrument on hepatic diseases of type IDSG ;Rat
Dou AX, Zong L,Zhou K, Chen T ,Pan H, Zhang Q,Li JQ,Fang JY,Lu LG.Experimental study cinnonalcoholic fatty liver disease treated by medical instrument on hepatic diseases of type I DSG in rats .Shijie Huaren Xiaohua Zazhi 2008;16(9):935-940
摘要
目的：初步观察DSG-Ⅰ型生物信息红外肝病治疗仪治疗大鼠非酒精性脂肪性肝病的疗效。
方法：♂SD大鼠50只随机分组，正常对照组20只喂饲普通饲料，模型对照组及近红外光治疗组共30只喂饲高脂饲料（0.88标准普通饲料＋0.10猪油＋0.02胆固醇）。治疗组自13wk始进行近红外线照射治疗，于20wk结束治疗并处死各组大鼠，测定血清ALT、AST、GGT、ALP、TP、TG、TC、LDL和HDL水平，同时观察肝组织学改变。
结果：20wk试验结束时，与正常对照组相比，模型对照组肝指数(t=8.65,p<0.01)、血清ALT(t=4.65,p<0.01)、AST(t=4.74,p<0.01)、ALP(t=3.56,p<0.01)、TP(t=6.35,p<0.01)、TC(t=8.41,p<0.01)、LDL(t=8.86,p<0.01) 水平显著升高，肝组织脂肪变性(z=-4.135,p<0.01)、
炎症(z=-3.752,p<0.01) 及纤维化程度 (z=-4.156,p<0.01)均较对照组程度重。与模型对照组相比，近红外光治疗组肝指数、血清ALT、GGT、ALP、TP、TG和TC水平均有所下降，但无统计学意义，而AST有明显降低(t=-2.404,p<0.05)；肝组织脂肪变及炎症程度较模型组轻，但无统计学意义，而肝纤维化程度较模型组减轻(z=-3.101,p<0.01)。
结论：DSG-Ⅰ型生物信息红外肝病治疗仪对大鼠高脂饮食非酒精性脂肪性肝病有一定的治疗作用。
关键词：非酒精性脂肪性肝病；DSG-Ⅰ型生物信息红外肝病治疗仪；大鼠
窦爱霞, 宗蕾，周琨，陈婷，潘浩，张权，李继强，房静远，陆伦根
DSG-Ⅰ型生物信息红外肝病治疗仪大鼠非酒精性脂肪肝.世界华人消化杂志2008;16(9)935-940
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